The white-clawed crayfish is the only native crayfish species in the UK. It is endangered and under threat from invasive crayfish species such as signal crayfish and the crayfish plague. Our simple to use eDNA test provides a cost-effective opportunity to monitor these species alongside other native and non-native crayfish species.
Our crayfish eDNA Analysis service can identify if a single or multiple crayfish species are present or absent within a waterbody such as a pond, stream or river. Our in house developed eDNA test for crayfish has been developed following industry standards to provide a reliable and accurate service.
Our Filter eDNA Collection Kit has been specially designed and optimised to obtain the maximum amount of DNA from a water sample. By using filters, we can collect DNA from volumes up to 1L (over 10x the amount collected using the ethanol filled tubes for GCN eDNA).
Only one Filter eDNA Collection Kit is needed per site, to detect up to four different target species. Mix and match to analyse multiple species such as: white-clawed crayfish, signal crayfish, marbled crayfish, crayfish plague, smooth newt, Asian clam… Basically any four species from our other species detectable page here.
Filter eDNA Collection Kit: £30 +vat
£140 +vat
£170 +vat
£200 +vat
£230 +vat
As standard, all results are provided within 10 working days from receiving the sample at the laboratory. Fast Track 5 working days (+£100 + vat per sample) may be available depending on lab capacity.
There is no need to pre-book standard analysis with SureScreen Scientifics, simply send your samples to us and we will email you to let you know when we have received them.
*** Please call ahead to check availability if wishing to use our 5 working day service for Crayfish analysis ***
The cost of analysis is invoiced to your account (for existing customers) and the results are made available when ready.
NB: New customers will need to pay for their analysis upfront before results are sent (for first order only)
Due to seasonal weather conditions and changes in crayfish activity over the year, sample collection is best suited to the summer months where crayfish activity levels are higher.
Like the white-clawed crayfish, signal crayfish are also less active in winter months. For best results sample during breeding or just after hatching.
Due to weather and seasonal activity cycles of crayfish, all our other detectable species such as red swamp and marbled crayfish are best sampled for in the summer months.
Detectable all year round due to the presence of spores, it is reccomended to avoid winter months when extreme weather conditions can cause a dilution of detecatble DNA.
01.
Our white-clawed crayfish eDNA service has been developed in house over a 4 year period, in collaboration with the University of Derby. Our assay has been designed using the most sensitive sample collection technique and validated in a wide range of habitats including ponds, streams, rivers and lakes.
02.
With each Filter eDNA Collection Kit able to detect up to four different species, feel free to add additional species such as freshwater pearl mussel, European eel and Atlantic salmon. See our eDNA analysis page for a full list of target species.
03.
We have introduced practices to increase sustainability and reduce waste such reduction in packaging matieal, investing in laboratory recycling schemes and switching to carbon neutral products in our sampling kits, in cases we cannot switch out the plastic.
04.
Fast Track 5 working days (+£100 + vat per sample) may be available depending on lab capacity. Please call ahead to check availability.
We recommend using a local courier service such as DPD Local who will collect from you directly and deliver to us next working day. All you need to do is make sure your samples are packaged ready for collection. You will simply need to enter the approximate weight of the parcel (GCN kit is 0.5 kg / Filter kit is 0.3kg) and the collection address. Booking online is quick and easy – CLICK HERE to book your collection.
Important: Please do not arrange collections on a Friday as your samples won’t reach us until Monday meaning they will be stored in unknown conditions over the weekend so it is best to keep collections to Mon-Thurs for optimal sample quality.
Please send to:
SureScreen Scientifics Ltd, Morley Retreat, Church Lane, Morley, Derbyshire, DE7 6DE.
We also accept hand-delivered kits between 08.00 and 16.00 Monday to Friday, and by appointment 24/7.
There is no need to pre-book standard analysis with SureScreen Scientifics, simply send your samples to us and we will email you to let you know when we have received them.
*** Please call ahead to check availability if wishing to use our 5 working day service for Crayfish analysis ***
This depends on the size of the site and the question that you are trying to answer. For a small isolated site such as a pond, small lake or isolated river site, one sample should be sufficient. For larger river systems and reservoirs, multiple samples should be taken from multiple sites to ensure the site is best represented by the sampling strategy. If you are struggling to determine how many samples to collect for a study site, then get in touch with us and we will be able to advise.
If you require a high level of scientific accuracy we recommend that you collect replicate samples from each site to ensure consistency and reliability in results.
Each sample can be analysed for up to four different eDNA target species currently offered by SureScreen. Once the DNA has been extracted from a sample it can then be analysed multiple times: i.e. for white-clawed crayfish, signal crayfish, marbled crayfish and the crayfish plague.
The assays used in the laboratory for the detection of the crayfish species and crayfish plague have by design been developed as species specific. This means that they will only detect and amplify DNA of the target species, thorough testing has been conducted to ensure that this is the case and that each of our assays for A. pallipes, P. leniusculus, P.virginalis, A. astaci and our other eDNA services do not cross-amplify any other species.
With the crayfish plague being a large problem for white-clawed crayfish in the UK at the moment, it is highly important that biosecurity measures are followed when collecting crayfish eDNA samples. We have designed a biosecurity friendly eDNA kit which includes single use components and therefore reduces the risk of transferring plague from site to site. However, it is also important that the end-user thoroughly cleans any additional equipment, wellies and clothes which they take to any site before moving onto a new site to reduce the risk of transferring any plague spores.
Anyone can sample! A license is only required if you are conducting a white-clawed crayfish survey additionally to the eDNA survey. If you are just simply taking a water sample and not intending to search for crayfish or disturb their habitat then you do not require a licence.
At the moment, this assay cannot be used on a legal basis to support planning and building applications. However, it can be a useful survey tool for the primary detection of populations as a complimentary survey technique.
This method is not currently approved by any approval body, however we are working towards this with the Environment Agency taking an assisting role with the validation of our method.
The crayfish eDNA has a similar accuracy to that of the GCN eDNA service. View the published research for more in-depth information.
We recommend that samples should be collected between mid-April and late-October to coincide with when crayfish are most active (and hence releasing most eDNA). Samples can be taken outside of this window but may not provide highly reliable or repeatable results. Further to this the sample should be collected when the pond/river/stream is relatively calm, with little turbidity. Try to avoid sample collection from murky rivers/ponds or at sites just after large rainfall as the filter will soon clog and you will be unable to pass a sufficient volume of water through for analysis.
The filters are designed to process up to 1 litre of fluid. However, in the case of rivers and streams, due to turbidity and sediment load it may not always be possible to filter such a high volume. We recommend the filtration of at least 150ml with an ideal filtered volume of 400ml, the higher the filtered volume the increased chance of obtaining eDNA within your sample (if it is present within the sample site). If you are unable to filter such high volumes – don’t worry. Just make a note of the volume which was filtered on the sample collection form.
One Filter eDNA Collection Kit is required for one pond up to approximately 1 hectare (2.5 acres). For ponds larger than this it is recommended to collect one kit per hectare. Generally, the most accepted way of collecting samples for a large pond of say 4 acres, is that two kits be used, one to collect 20 sub-samples around one half of the pond and the other kit to collect 20 sub-samples around the other half of the pond. These kits are analysed separately (and therefore charged as two kits and two analyses) as to not dilute the sample and give best chance to detect eDNA.
Small Streams & Rivers (less than 10m wide/less than 30cm deep)
Where possible try to avoid entering the water system and collect samples from either side of the bank. If it is necessary and safe to enter the water system, collect samples following a zig-zag pattern. Ensure that you sample in an upstream direction to avoid the collection of ancient sediment which may contain historical DNA from the target species. If you enter the watercourse, ensure that you implement full biosecurity protocols to avoid the potential spread of the crayfish plague.
Large Rivers
For larger more difficult to access rivers it may be necessary to use multiple collection kits in order to ensure the reliability of species presence/absence. If you are unable to enter the watercourse as in the methods above then try where possible to collect samples from the edges, collecting a sub-sample every few metres in order to get a representative sample from the site.
If studying a large river system, it may be necessary to collect multiple samples at multiple points. For example, the collection of a sample every 500-1000m within a river. This is to ensure that you identify any populations which may be fragmented within the stream. Collecting multiple samples also enables the user to accurately indicate where a population may lie within the stream, as opposed to only collecting one sample and only knowing if the species is present or absent.
Key Advice
Results indicate that the DNA levels drop below detectable amounts no later than one month after all individuals have left the system. This means that there is a minimal chance of detecting a population of crayfish which has been absent for several months.
eDNA should not be used as a replacement for established/traditional survey methods. In its present form it is designed to be complimentary to these methods, as a primary survey technique which can enable the screening of large areas in a short amount of time with minimal training and licensing needs.
We recommend using a local courier service such as DPD Local who will collect from you directly and deliver to us next working day. All you need to do is make sure your samples are packaged ready for collection. You will simply need to enter the approximate weight of the parcel (GCN kit is 0.5 kg / Filter kit is 0.3kg) and the collection address. Booking online is quick and easy – CLICK HERE to book your collection.
Important: Please do not arrange collections on a Friday as your samples won’t reach us until Monday meaning they will be stored in unknown conditions over the weekend so it is best to keep collections to Mon-Thurs for optimal sample quality.
Please send to:
SureScreen Scientifics Ltd, Morley Retreat, Church Lane, Morley, Derbyshire, DE7 6DE.
We also accept hand-delivered kits between 08.00 and 16.00 Monday to Friday, and by appointment 24/7.
There is no need to pre-book standard analysis with SureScreen Scientifics, simply send your samples to us and we will email you to let you know when we have received them.
*** Please call ahead to check availability if wishing to use our 5 working day service for Crayfish analysis ***
